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Product Name
Histone H4 Polyclonal Antibody
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Description
Polyclonal antibody to Histone H4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HIST2H4B antibody; H4/o antibody; histone H4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human HIST2H4B (NP_001029249.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - Histone H4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using Histone H4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - Histone H4 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using Histone H4 antibody . Blue: DAPI for nuclear staining.
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"