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Product Name
Histone H4 antibody
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Description
Histone H4 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse thymus tissue, HeLa cells, HepG2 cells, HT-1080 cells, MCF7 cells, mouse kidney tissue, rat thymus tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 14 kDa and 11 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H4/A antibody; H4/B antibody; H4/C antibody; H4/D antibody; H4/E antibody; H4/G antibody; H4/H antibody; H4/I antibody; H4/j antibody; H4/K antibody; H4/M antibody; H4/N antibody; H4/O antibody; H4F2 antibody; H4FA antibody; H4FB antibody; H4FC antibody; H4FD antibody; H4FE antibody; H4FG antibody; H4FH antibody; H4FI antibody; H4FJ antibody; H4FK antibody; H4FM antibody; H4FN antibody; H4FO antibody; HIST1H4A antibody; HIST1H4B antibody; HIST1H4C antibody; HIST1H4D antibody; HIST1H4E antibody; HIST1H4F antibody; HIST1H4H antibody; HIST1H4I antibody; HIST1H4J antibody; HIST1H4K antibody; HIST1H4L antibody; HIST2H4 antibody; HIST2H4A antibody; HIST2H4B antibody; HIST4H4 antibody; histone cluster 1 antibody; H4e antibody; Histone H4 antibody; histoneh4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Histone H4 recombinant protein (Accession Number: BC012587). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:111408(HIST1H4E antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111408(HIST1H4E antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111408(HIST1H4E antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Histone H4 (IP:Catalog No:111408, 3ug; Detection:Catalog No:111408 1:600) with HeLa cells lysate 2000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:111408(Histone H4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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References
- Gong K, Xie J, Yi H, Li W. CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1 arrest, ROS-dependent apoptosis and differentiation in human leukaemia cells. The Biochemical journal. 443(3):735-46. 2012.
- Kim JA, Hsu JY, Smith MM, Allis CD. Mutagenesis of pairwise combinations of histone amino-terminal tails reveals functional redundancy in budding yeast. Proceedings of the National Academy of Sciences of the United States of America. 109(15):5779-84. 2012.
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