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Product Name
Histone H3.3 antibody
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Description
Histone H3.3 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse thymus tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 15-17 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H3 histone antibody; family 3A antibody; H3.3A antibody; H3.3B antibody; H3F3 antibody; H3F3A antibody; H3F3B antibody; Histone H3.3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Histone H3.3 recombinant protein (Accession Number: NM_001379046). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:111407(H3F3A antibody) at dilution of 1:400
Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using Catalog No:111407(Histone H3.3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. [PMID: 8586426] Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. H3F3A is one replacement histone [PMID:16258499].
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