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Histone H2B antibody

Cat.#: 111406

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Product Information

  • Product Name
    Histone H2B antibody
  • Documents
  • Description
    Histone H2B Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human pancreas tissue, human breast cancer tissue.
  • Tested applications
    ELISA, IHC, WB, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    GL105 antibody; H2B antibody; H2B.1 antibody; H2B/q antibody; H2BFQ antibody; HIST2H2BE antibody; histone cluster 2 antibody; H2be antibody; Histone H2B antibody; Histone H2B GL105 antibody; Histone H2B type 2 E antibody; Histone H2B.q antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Histone H2B recombinant protein (Accession Number: NM_003528). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Immunohistochemical of paraffin-embedded human pancreas using Catalog No:111406(HIST2H2BE antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human pancreas using Catalog No:111406(HIST2H2BE antibody) at dilution of 1:100 (under 40x lens)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111406(Histone H2B Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111406(Histone H2B Antibody) at dilution of 1:1000

    IP Result of anti-Histone H2B (IP:Catalog No:111406, 4ug; Detection:Catalog No:111406 1:500) with HEK-293 cells lysate 2000ug.

    IP Result of anti-Histone H2B (IP:Catalog No:111406, 4ug; Detection:Catalog No:111406 1:500) with HEK-293 cells lysate 2000ug.

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:111406(Histone H2B Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:111406(Histone H2B Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    HIST2H2BE represents histone cluster 2, H2be
  • References
    • Whitcomb SJ, Fierz B, McGinty RK. Histone monoubiquitylation position determines specificity and direction of enzymatic cross-talk with histone methyltransferases Dot1L and PRC2. The Journal of biological chemistry. 287(28):23718-25. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"