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Product Name
hIST1 antibody
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Description
hIST1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human hepat℃irrhosis tissue. Positive WB detected in mouse liver tissue, HeLa cells, mouse brain tissue, PC-3 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hIST1 antibody; IST1 antibody; IST1 homolog antibody; KIAA0174 antibody; OLC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of hIST1 recombinant protein (Accession Number: NM_001270977). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111358(hIST1 Antibody) at dilution of 1:1000
IP Result of anti-hIST1 (IP:Catalog No:111358, 4ug; Detection:Catalog No:111358 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:111358(hIST1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111358(hIST1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:111358(hIST1 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KIAA0174 represents KIAA0174
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"