HIRA Polyclonal Antibody

Cat.#: 165892

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Product Information

  • Product Name
    HIRA Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to HIRA
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse
  • Alternative names
    HIRA antibody; DGCR1 antibody; TUP1 antibody; TUPLE1 antibody; protein HIRA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:100 - 1:200
  • Validations

    Western blot - HIRA Polyclonal Antibody

    Western blot - HIRA Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using HIRA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human gastric cancer using HIRA antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human lung cancer using HIRA antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry - HIRA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human colon carcinoma using HIRA antibody at dilution of 1:100 (40x lens).

  • Background
    Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"