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Product Name
HIC5 antibody
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Description
HIC5 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IP detected in A431 cells. Positive IHC detected in human spleen tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 48kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARA55 antibody; HIC 5 antibody; HIC5 antibody; TGFB1I1 antibody; TSC 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HIC5 recombinant protein (Accession Number: NM_001164719). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111344(TGFB1I1 antibody) at dilution of 1:1000
IP Result of anti-TGFB1I1 (IP:Catalog No:111344, 4ug; Detection:Catalog No:111344 1:500) with A431 cells lysate 1520ug.
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:111344(TGFB1I1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:111344(TGFB1I1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:111344(TGFB1I1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TGFB1I1 represents transforming growth factor beta 1 induced transcript 1
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