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Product Name
HFM1 Polyclonal Antibody
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Description
Polyclonal antibody to HFM1
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
HFM1 antibody; MER3 antibody; POF9 antibody; SEC63D1 antibody; Si-11 antibody; Si-11-6 antibody; helicase antibody; probable ATP-dependent DNA helicase HFM1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - HFM1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HFM1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
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Background
Required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"