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Product Name
HEYL antibody
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Description
HEYL Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in MDA-MB-453s cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bHLHb33 antibody; HEY3 antibody; HEYL antibody; hHeyL antibody; hHRT3 antibody; HRT 3 antibody; HRT3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HEYL recombinant protein (Accession Number: NM_014571). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using HEYL antibody Catalog No:111310 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:111310(HEYL Antibody) at dilution of 1:600
IP Result of anti-HEYL (IP:Catalog No:111310, 4ug; Detection:Catalog No:111310 1:300) with HeLa cells lysate 2600ug.
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Background
HEYL represents hairy/enhancer-of-split related with YRPW motif-like
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"