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Product Name
HEY2 Polyclonal Antibody
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Description
Polyclonal antibody to HEY2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
HEY2 antibody; CHF1 antibody; GRIDLOCK antibody; GRL antibody; HERP1 antibody; HESR2 antibody; HRT2 antibody; bHLHb32 antibody; hairy/enhancer-of-split related with YRPW motif protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HEY2 (NP_036391.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - HEY2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HEY2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"