HEXIM1 Peptide (Pen-BR)

HEXIM1 Peptide (Pen-BR)

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-RQIKIWFQNRRWGGQLGKKKHRRRPSKKKRHW-OH

H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Trp-Gly-Gly-Gln-Leu-Gly-Lys-Lys-Lys-His-Arg-Arg-Arg-Pro-Ser-Lys-Lys-Lys-Arg-His-Trp-OH

32

95.2%

C₁₈₉H₃₀₇N₇₁O₃₈

4181.9

Synthetic

Powder

To overcome the poor cell penetrating ability of HEXIM1 peptides, we fused both HEXIM1 BR and BR-RRR12 peptides with a cell penetrating peptide, Pen, to generate Pen-BR and Pen-RRR peptides. Pen peptide was derived from the original Penetratin cell penetrating peptide and was used in an earlier study as a cell penetrating peptide for E. coli and S. aureus (Schmidt et al., 2014). Both Pen-BR and Pen-RRR peptides showed improved and potent bacterial inhibitory (MIC values between 4 and 16 mM) and killing activities (MBC values between 8 and 32 mM).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Ho PL, Ong HK, Teo J, Ow DS, Chao SH. HEXIM1 Peptide Exhibits Antimicrobial Activity Against Antibiotic Resistant Bacteria Through Guidance of Cell Penetrating Peptide. Front Microbiol. 2019

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.