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Product Name
HERP antibody
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Description
HERP Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, SW 1990 cells. Positive IHC detected in human lung cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
HERP antibody; HERPUD1 antibody; KIAA0025 antibody; Mif1 antibody; SUP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HERP recombinant protein (Accession Number: NM_014685). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111298(HERPUD1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111298(HERPUD1 antibody) at dilution of 1:100 (under 10x lens)
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Background
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson’s disease, levodopa-related dyskinesias and Tardive dyskinesia.
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References
- Hao L, Vassena R, Wu G. The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome. Biology of reproduction. 80(5):944-53. 2009.
- Meir O, Dvash E, Werman A, Rubinstein M. C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models. PloS one. 5(3):e9516. 2010.
- Zhuo JM, Kruger WD, Praticò D. The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia. Journal of Alzheimer's disease : JAD. 20(2):569-76. 2010.
- Tuusa JT, Petäjä-Repo UE. Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling. Molecular and cellular biochemistry. 351(1-2):173-81. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"