Human HCST / DAP10 (Fc Tag) recombinant protein

Human HCST / DAP10 (Fc Tag) recombinant protein

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Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.

Hematopoietic cell signal transducer

PIK3AP; PHOSPHOINOSITIDE-3-KINASE ADAPTOR PROTEIN; DAP10; UNQ587/PRO1157; MEMBRANE PROTEIN DAP10; KINASE ASSOC PRO OF ; HCST; KINASE ASSOC PROTEIN; KAP10; 10KDA; HEMATOPOIETIC CELL SIGNAL TRANSDUCER; DNAX-ACTIVATION PROTEIN 10; TRANSMEMBRANE ADAPTER PROTEIN KAP10

Q9UBK5

HCST; DAP10; KAP10; PIK3AP; UNQ587/PRO1157

Human Cells

A DNA sequence encoding the human HCST (Q9UBK5-1) (Met-Pro48) was fused with Fc region of mouse IgG at the C-terminus.

Human

The recombinant human HCST/mFc is a disulfide-linked homodimer. The reduced monomer comprises 264 amino acids and has a predicted molecular mass of 29.3 kDa. The apparent molecular mass of the protein is approximately 34 in SDS-PAGE under reducing conditions.

(67.3+28) % as determined by SDS-PAGE