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Product Name
HDLBP antibody
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Description
HDLBP Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, Jurkat cells. Positive IP detected in Jurkat cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 141kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ16432 antibody; HBP antibody; HDL binding protein antibody; HDLBP antibody; PRO2900 antibody; VGL antibody; Vigilin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HDLBP recombinant protein (Accession Number: XM_011511060). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111390(HDLBP antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells, using HDLBP antibody Catalog No:111390 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HDLBP (IP:Catalog No:111390, 4ug; Detection:Catalog No:111390 1:500) with Jurkat cells lysate 2400ug.
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Background
HDLBP represents high density lipoprotein binding protein
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References
- Zhao C, Zhang W, Tian X. Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis. Journal of medical virology. 82(1):14-22. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"