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Product Name
HDGFRP3 antibody
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Description
HDGFRP3 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in human brain tissue, Y79 cells. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 142 antibody; HDGF 2 antibody; HDGF2 antibody; HDGFRP3 antibody; HRP 3 antibody; HRP-3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HDGFRP3 recombinant protein (Accession Number: NM_016073). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111387(HDGFRP3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111387(HDGFRP3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using HDGFRP3 antibody Catalog No:111387 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-HDGFRP3 (IP:Catalog No:111387, 3ug; Detection:Catalog No:111387 1:300) with mouse testis tissue lysate 4800ug.
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Background
HDGFRP3, also named as Hepatoma-derived growth factor-related protein 3 or HDGF2, is a 203 amino acid protein, which contains 1 PWWP domain and belongs to the HDGF family. HDGFRP3 detected in testis, heart, spinal cord and brain. HDGFRP3 as a nuclear potein enhances DNA synthesis and may play a role in cell proliferation.
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References
- Yun HS, Hong EH, Lee SJ. Depletion of hepatoma-derived growth factor-related protein-3 induces apoptotic sensitization of radioresistant A549 cells via reactive oxygen species-dependent p53 activation. Biochemical and biophysical research communications. 439(3):333-9. 2013.
- Yun HS, Baek JH, Yim JH. Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation. Biochemical and biophysical research communications. 449(4):471-6. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"