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Product Name
HDAC4-specific antibody
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Description
HDAC4-specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 119-140 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HA6116 antibody; HD4 antibody; HDAC A antibody; HDAC4 antibody; HDACA antibody; histone deacetylase 4 antibody; KIAA0288 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: BC039904). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111378(HDAC4-specific Antibody) at dilution of 1:600
IP Result of anti-HDAC4-specific (IP:Catalog No:111378, 4ug; Detection:Catalog No:111378 1:500) with HeLa cells lysate 2440ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111378(HDAC4-specific Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:111378(HDAC4-specific Antibody) at dilution of 1:50 (under 40x lens)
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Background
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4.
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References
- Yuan JH, Yang F, Chen BF. The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma. Hepatology (Baltimore, Md.). 54(6):2025-35. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"