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Product Name
HDAC1-specific antibody
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Description
HDAC1-specific Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse testis tissue, mouse brain tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686H12203 antibody; GON 10 antibody; HD1 antibody; HDAC1 antibody; histone deacetylase 1 antibody; RPD3 antibody; RPD3L1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004964). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111372(HDAC1-specific antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells using Catalog No:111372(HDAC1-specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is speicifc to HDAC1. It does not react with other HDACs.
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