-
Product Name
hD53; TPD52L1 antibody
- Documents
-
Description
hD53; TPD52L1 Rabbit Polyclonal antibody. Positive IHC detected in human prostate tissue. Positive IF detected in MCF-7 cells. Positive WB detected in MCF7 cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 25 kDa
-
Tested applications
ELISA, IHC, WB, IP, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
D53 antibody; hD53 antibody; TDP53 antibody; TPD52L1 antibody; TPD52L2 antibody; TPD53 antibody; tumor protein D52 like 1 antibody; Tumor protein D53 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of hD53; TPD52L1 recombinant protein (Accession Number: NM_001003396). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111369(hD53; TPD52L1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate using Catalog No:111369(hD53; TPD52L1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate using Catalog No:111369(hD53; TPD52L1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-hD53; TPD52L1 (IP:Catalog No:111369, 3ug; Detection:Catalog No:111369 1:300) with MCF-7 cells lysate 2500ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:111369(hD53; TPD52L1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"