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Product Name
HCRTR1 Polyclonal Antibody
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Description
Polyclonal antibody to HCRTR1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HCRTR1 antibody; OX1R antibody; hypocretin receptor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - HCRTR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HCRTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunofluorescence - HCRTR1 Polyclonal Antibody
Immunofluorescence analysis of rat brain using HCRTR1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"