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Product Name
HBXIP antibody
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Description
HBXIP Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human hepat℃irrhosis tissue, human breast cancer tissue, human heart tissue, human liver cancer tissue. Positive WB detected in Transfected HEK-293 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
HBV X interacting protein antibody; HBX interacting protein antibody; HBXIP antibody; XIP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HBXIP recombinant protein (Accession Number: NM_006402). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:111275(HBXIP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:111275(HBXIP antibody) at dilution of 1:50 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111275(HBXIP Antibody) at dilution of 1:800
1X10^6 MCF-7 cells were stained with 0.2ug HBXIP antibody (Catalog No:111275, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
HBXIP, also named as XIP, belongs to the HBXIP family. It is originally identified by its interaction with the C-terminus of hepatitis B virus (HBV) X protein (HBx). HBXIP is a regulator of centrosome dynamics and cytokinesis. HBXIP is able to up-regulate S100A4 though activating STAT4 and inducing DNA methylation of PTEN. HBXIP is a cellular 18kd protein and cytoplasm stain.
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References
- Xu F, You X, Liu F. The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells. Cancer letters. 333(1):124-32. 2013.
- Liu S, Li L, Zhang Y. The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. The Journal of biological chemistry. 287(36):30228-39. 2012.
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