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Product Name
HBS1L antibody
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Description
HBS1L Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in Jurkat cells, A2780 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 75 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686L13262 antibody; EF 1a antibody; ERFS antibody; HBS1 antibody; HBS1 like (S. cerevisiae) antibody; HBS1 like protein antibody; HBS1L antibody; HSPC276 antibody; KIAA1038 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HBS1L recombinant protein (Accession Number: NM_006620). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111274(HBS1L antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111274(HBS1L antibody) at dilution of 1:50 (under 25x lens)
Immunofluorescent analysis of HepG2 cells, using HBS1L antibody Catalog No:111274 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HBS1L (IP:Catalog No:111274, 4ug; Detection:Catalog No:111274 1:1000) with mouse brain tissue lysate 4000ug.
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Background
HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.
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References
- Slevin MK, Meaux S, Welch JD. Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes. Molecular cell. 53(6):1020-30. 2014.
- Knight JR, Bastide A, Roobol A. Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. The Biochemical journal. 465(2):227-38. 2015.
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