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Product Name
HBA1-Specific antibody
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Description
HBA1-Specific Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human spleen tissue. Observed molecular weight by Western-blot: 14-16kd
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Tested applications
ELISA, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Alpha globin antibody; HBA1 antibody; HBA2 antibody; Hemoglobin alpha chain antibody; Hemoglobin subunit alpha antibody; hemoglobin antibody; alpha 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000558). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111265(HBA1-Specific antibody) at dilution of 1:300
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Background
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.
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