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Product Name
HAUS3 antibody
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Description
HAUS3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 62-65 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C4orf15 antibody; HAUS3 antibody; IT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_024511). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111261(C4orf15 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111261(C4orf15 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111261(C4orf15 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:111261(C4orf15 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.
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