HAT1 antibody

Cat.#: 111260

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Product Information

  • Product Name
    HAT1 antibody
  • Documents
  • Description
    HAT1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive WB detected in MCF7 cells, human liver tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 46kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HAT1 antibody; histone acetyltransferase 1 antibody; KAT1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HAT1 recombinant protein (Accession Number: BC018682). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111260(HAT1 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111260(HAT1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111260(HAT1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111260(HAT1 antibody) at dilution of 1:50 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using HAT1 antibody Catalog No:111260 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using HAT1 antibody Catalog No:111260 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-HAT1 (IP:Catalog No:111260, 4ug; Detection:Catalog No:111260 1:500) with HepG2 cells lysate 3200ug.

    IP Result of anti-HAT1 (IP:Catalog No:111260, 4ug; Detection:Catalog No:111260 1:500) with HepG2 cells lysate 3200ug.

  • Background
    HAT1 represents histone acetyltransferase 1
  • References
    • Yu H, Diao H, Wang C. Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Molecular & cellular proteomics : MCP. 14(4):1009-23. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"