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Product Name
HAND2 Polyclonal Antibody
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Description
Polyclonal antibody to HAND2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HAND2 antibody; DHAND2 antibody; Hed antibody; Thing2 antibody; bHLHa26 antibody; dHand antibody; heart and neural crest derivatives expressed 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human HAND2 (NP_068808.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - HAND2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HAND2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - HAND2 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using HAND2 antibody . Blue: DAPI for nuclear staining.
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Background
Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3' (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"