Halocidin peptide

Halocidin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

WLNALLHHGLNCAKGVLA

H-Trp-Leu-Asn-Ala-Leu-Leu-His-His-Gly-Leu-Asn-Cys-Ala-Lys-Gly-Val-Leu-Ala-NH2

18

97.1%

C₈₈H₁₄₁N₂₇O₂₀S

1928.3

Synthetic

Powder

Shown is the sequence of chain A. The sequence of chain B is ALLHHGLNCAKGVLA, which is an N-terminally 3-residue truncated version of the chain A. AMP stability: Shin YP et al. (2010 Antimicrob Agents Chemother 54:2855-66) showed that appending a Lys at the N-terminus of chain A and making chain B identical to the new chain A, leading to a peptide called HG1 that is resistant to proteases such as trypsin, chymotrypsin, and MMP-7. Animal model:mouse: A single intravenous injection of the modified chain A by itself into mice resulted in an significant therapeutic effect (Jang et al. 2007 ibid 51: 4148-56). An HG1 isomer with two substitutions of Gln-3 and Lys-19, named haloganan, showed an advantage over HG1 in that it exerted its potent antimicrobial activity under conditions containing human serum and/or wound fluid (Shin et al., 2014). Updated 10/8/2010; 12/2014 GW. Halocidin has Antibacterial activity. The source of Halocidin is hemocytes, Halocynthia aurantium.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Jang WS, Kim CH, Kang MS, et al. cDNA cloning of halocidin and a new antimicrobial peptide derived from the N-terminus of Ci-META4. Peptides. 2005;26(12):2360-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.