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Product Name
HADHB Polyclonal Antibody
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Description
Polyclonal antibody to HADHB
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HADHB antibody; ECHB antibody; MSTP029 antibody; MTPB antibody; TP-BETA antibody; trifunctional enzyme subunit beta, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-270 of human HADHB (NP_000174.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - HADHB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HADHB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - HADHB Polyclonal Antibody
Immunofluorescence analysis of A549 cells using HADHB antibody . Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"