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Product Name
HADHA antibody
- Documents
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Description
HADHA Mouse Monoclonal antibody. Positive WB detected in A375 cells, HepG2 cells, Jurkat cells. Positive IHC detected in human heart tissue, human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 79 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
78 kDa gastrin binding protein antibody; ECHA antibody; HADH antibody; HADHA antibody; LCHAD antibody; MTPA antibody; TP ALPHA antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of HADHA recombinant protein (Accession Number: NM_000182). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107311(HADHA antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:107311(HADHA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107311(HADHA antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107311(HADHA Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).
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References
- Du Y, Meng Y, Zhu J. Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice. Proteomics. 14(17-18):2072-83. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"