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Product Name
HADH antibody
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Description
HADH Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human hepat℃irrhosis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HAD antibody; HADH antibody; HADH1 antibody; HADHSC antibody; HCDH antibody; HHF4 antibody; M/SCHAD antibody; SCHAD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HADH recombinant protein (Accession Number: NM_005327). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111332(HADH antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111332(HADH antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111332(HADH antibody) at dilution of 1:400 (under 40x lens)
IP Result of anti-HADH (IP:Catalog No:111332, 3ug; Detection:Catalog No:111332 1:700) with HepG2 cells lysate 400ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:111332(HADH Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.
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References
- Martin J, Maurhofer O, Bellance N. Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function. Hepatology (Baltimore, Md.). 57(5):2037-48. 2013.
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