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Product Name
HA tag antibody
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Description
HA tag Rabbit Polyclonal antibody. Positive WB detected in recombinant protein, Transfected HEK-293 cells. Positive IP detected in Transfected HEK-293 cells. Positive IF detected in Transfected HEK-293 cells.
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Tag
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Alternative names
CYPYDVPDYA antibody; HA tag antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide. Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:1000-1:10000
IF: 1:10-1:100
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Validations
Western blot of HA-tagged fusion protein with anti-HA-tag (Catalog No:117324) at various dilutions.
IP Result of anti-HA-tag (IP:Catalog No:117324, 3ug; Detection:Catalog No:117324 1:2000) with Transfected HEK-293 cells lysate 500ug.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117324(HA-tag Antibody) at dilution of 1:3000
Immunofluorescent analysis of Transfected HEK-293 cells using Catalog No:117324(HA-tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
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References
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- Xu LR, Liu XL, Chen J, Liang Y. Protein disulfide isomerase interacts with tau protein and inhibits its fibrillization. PloS one. 8(10):e76657. 2013.
- Qiu Y, Wang Z, Liu Y. The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids. PloS one. 9(2):e89628. 2014.
- Sun X, Lu B, Hu B, Xiao W, Li W, Huang Z. Novel function of the chromosome 7 open reading frame 41 gene to promote leukemic megakaryocyte differentiation by modulating TPA-induced signaling. Blood cancer journal. 4:e198. 2014.
- Xie HX, Lu JF, Rolhion N. Edwardsiella tarda-Induced cytotoxicity depends on its type III secretion system and flagellin. Infection and immunity. 82(8):3436-45. 2014.
- Chen Z, Zhu M, Pan X. Inhibition of Hepatitis B virus replication by SAMHD1. Biochemical and biophysical research communications. 450(4):1462-8. 2014.
- Qiu Y, Miao M, Wang Z. The RNA binding of protein A from Wuhan nodavirus is mediated by mitochondrial membrane lipids. Virology. 462-463:1-13. 2014.
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