H-Tyr-Cys-Trp-Ser-Gln-Tyr-Leu-Cys-Tyr-OH peptide

H-Tyr-Cys-Trp-Ser-Gln-Tyr-Leu-Cys-Tyr-OH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

YCWSQYLCY

H-Tyr-Cys-Trp-Ser-Gln-Tyr-Leu-Cys-Tyr-OH

9

97.8%

C₅₈H₇₁N₁₁O₁₅S₂

1226.4

Synthetic

Powder

This TNF-alpha antagonist inhibited TNF-alpha mediated apoptosis and exhibited an IC50 of 5 RM in the binding assay. It is the smallest peptidomimetic developed to date that might be used as a lead compound for the next generation of non-peptidic inhibitors. The name WP9QY arises from the binding to the site observed in the TNF-β/TNF-receptor(I) complex designated WP9 and the introduction of the amino acids Gln and Tyr.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• W.Takasaki et al., Nat. Biotechnol., 15, 1266 (1997)
• T.Kojima et al., J. Med. Dent. Sci., 52, 91 (2005)
• H.Saito et al., Arthritis Rheum, 56, 1164 (2007)
• K.Aoki, J. Oral Biosci., 51, 119 (2009)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.