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Product Name
GZMB antibody
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Description
GZMB Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 33kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CCPI antibody; CGL 1 antibody; CGL1 antibody; CSP B antibody; CSPB antibody; CTLA1 antibody; CTSGL1 antibody; Endogenous granzyme B antibody; Granzyme B antibody; GZMB antibody; HLP antibody; SECT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GZMB recombinant protein (Accession Number: NM_004131). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:111322(GZMB antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111322(GZMB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111322(GZMB antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using GZMB antibody Catalog No:111322 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization(PMID:17283187).GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA(PMID:11331782).This full length protein has 2 glycosylation sites and a signal peptide.Unglycosylated human granzyme B is 26 kDa and g high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei(PMID:8626751).
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References
- Li P, Zheng G, Yang Y. Granzyme B is recovered by natural killer cells via clathrin-dependent endocytosis. Cellular and molecular life sciences : CMLS. 67(18):3197-208. 2010.
- Cho YT, Lin JW, Chen YC. Generalized bullous fixed drug eruption is distinct from Stevens-Johnson syndrome/toxic epidermal necrolysis by immunohistopathological features. Journal of the American Academy of Dermatology. 70(3):539-48. 2014.
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