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Product Name
GUCY2C Polyclonal Antibody
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Description
Polyclonal antibody to GUCY2C
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
GUCY2C antibody; DIAR6 antibody; GC-C antibody; GUC2C antibody; MECIL antibody; MUCIL antibody; STAR antibody; guanylate cyclase 2C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-330 of human GUCY2C (NP_004954.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - GUCY2C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GUCY2C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
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Background
Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"