Human GCAP1 / GUCA1A recombinant protein

Human GCAP1 / GUCA1A recombinant protein

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• Material Safety Data Sheets (MSDS)

GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP 1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.

Guanylyl cyclase-activating protein 1

GCAP1; GUANYLYL CYCLASE-ACTIVATING PROTEIN 1; MGCAP1; GUANYLATE CYCLASE ACTIVATOR 1A (RETINA); CORD14; C6ORF131; CONE DYSTROPHY 3; PHOTORECEPTOR 1; GUCA1A; GCAP; RP1-139D8.2; GC-A; GUANYLIN 1; GCAP 1; DJ139D8.6; COD3; RETINA; GUANYLATE CYCLASE-ACTIVATING PROTEIN; GUCA1; GUCA

P43080

GUCA1A; C6orf131; GCAP; GCAP1; GUCA1

Baculovirus-Insect Cells

A DNA sequence encoding the human GUCA1A (P43080)(Met1-Gly201) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

Human

The secreted recombinant human GUCA1A consists of 203 amino acids and predicts a molecular mass of 23.1 KDa. The apparent molecular mass of the protein is approximately 20 KDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE