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  • GTF2E2 Polyclonal Antibody

GTF2E2 Polyclonal Antibody

Cat.#: 166641

Special Price 174.2 USD

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Product Information

  • Product Name
    GTF2E2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to GTF2E2
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    GTF2E2 antibody; FE antibody; TF2E2 antibody; TFIIE-B antibody; TTD6 antibody; general transcription factor IIE subunit 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human GTF2E2 (NP_002086.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
  • Validations

    Western blot - GTF2E2 Polyclonal Antibody

    Western blot - GTF2E2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using GTF2E2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

  • Background
    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"