-
Product Name
GTF2A2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to GTF2A2
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
GTF2A2 antibody; HsT18745 antibody; T18745 antibody; TF2A2 antibody; TFIIA antibody; TFIIA-12 antibody; TFIIA-gamma antibody; TFIIAS antibody; general transcription factor IIA subunit 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-109 of human GTF2A2 (NP_004483.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - GTF2A2 Polyclonal Antibody
Western blot analysis of extracts of NCI-H460 cells, using GTF2A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"