Product Name

GSTP1 antibody

Documents

Description

GSTP1 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human skin tissue, human liver tissue. Positive WB detected in Jurkat cells, COLO 320 cells, human brain tissue, K-562 cells, mouse brain tissue, PC-3 cells, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 23-28kd

Tested applications

ELISA, WB, IHC, IF, IP, FC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DFN7 antibody; FAEES3 antibody; Glutathione S transferase P antibody; glutathione S transferase pi 1 antibody; GST class pi antibody; GST3 antibody; GSTP1 antibody; GSTP1 1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of GSTP1 recombinant protein (Accession Number: NM_000852). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111191(GSTP1 antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human skin using Catalog No:111191(GSTP1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human skin using Catalog No:111191(GSTP1 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:111191 (GSTP1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-GSTP1 (IP:Catalog No:111191, 3ug; Detection:Catalog No:111191 1:800) with mouse brain tissue lysate 4000ug.

1X10^6 HepG2 cells were stained with 0.2ug GSTP1 antibody (Catalog No:111191, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Background

GSTP1 represents glutathione S-transferase pi 1

References

Cologna SM, Jiang XS, Backlund PS. Quantitative proteomic analysis of Niemann-Pick disease, type C1 cerebellum identifies protein biomarkers and provides pathological insight. PloS one. 7(10):e47845. 2012.
Chang SH, Jung IS, Han GY, Kim NH, Kim HJ, Kim CW. Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease. Biochemical and biophysical research communications. 430(2):670-5. 2013.
Yu H, Wark L, Ji H. Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice. Molecular nutrition & food research. 57(7):1158-69. 2013.
Song J, Zhao M, Liu X, Zhu Y, Hu X, Chen F. Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 58:306-10. 2013.
Shirai M, Arakawa S, Miida H. Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice. Journal of toxicologic pathology. 26(2):175-86. 2013.
Rojanathammanee L, Rakoczy S, Brown-Borg HM. Growth hormone alters the glutathione S-transferase and mitochondrial thioredoxin systems in long-living Ames dwarf mice. The journals of gerontology. Series A, Biological sciences and medical sciences. 69(10):1199-211. 2014.
Rojanathammanee L, Rakoczy S, Kopchick J, Brown-Borg HM. Effects of insulin-like growth factor 1 on glutathione S-transferases and thioredoxin in growth hormone receptor knockout mice. Age (Dordrecht, Netherlands). 36(4):9687. 2014.
Conklin DJ, Haberzettl P, Jagatheesan G. Glutathione S-transferase P protects against cyclophosphamide-induced cardiotoxicity in mice. Toxicology and applied pharmacology. 285(2):136-48. 2015.

GSTP1 antibody

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