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Product Name
GST Tag antibody
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Description
GST Tag Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein.
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Tested applications
ELISA, WB
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Species reactivity
Tag
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Alternative names
Glutathione S-Transferase antibody; GST antibody; gst tag antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Recombinant Protein (Accession Number: FN315687). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
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Validations
Western blot of GST-tagged fusion protein with anti-GST-tag (Catalog No:117323) at various dilutions.
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Background
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein.
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References
- He SQ, Zhang ZN, Guan JS. Facilitation of μ-opioid receptor activity by preventing δ-opioid receptor-mediated codegradation. Neuron. 69(1):120-31. 2011.
- Yang Y, Wang B, Yang D, Lu M, Xu Y. Prokaryotic expression of woodchuck cytotoxic T lymphocyte antigen 4 (wCTLA-4) and preparation of polyclonal antibody to wCTLA-4. Protein expression and purification. 81(2):181-5. 2012.
- Luo B, Zou T, Lu N. Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cDNA library. Biochemical and biophysical research communications. 416(1-2):39-44. 2011.
- Wang JX, Zeng Q, Chen L. SPINDLIN1 promotes cancer cell proliferation through activation of WNT/TCF-4 signaling. Molecular cancer research : MCR. 10(3):326-35. 2012.
- Fei X, Gu X, Fan S. Crystal structure of Human ASB9-2 and substrate-recognition of CKB. The protein journal. 31(4):275-84. 2012.
- Xiong W, Luo Y, Zhang C, Tan D, Zuo S. Expression, purification of recombinant human mitochondrial transcription termination factor 3 (hMTERF3) and preparation of polyclonal antibody against hMTERF3. Applied biochemistry and biotechnology. 167(8):2318-29. 2012.
- Song J, Ren H, Li Y. rG17PE38, a novel immunotoxin target to gastric cancer with overexpressed CCK-2R. Journal of drug targeting. 21(4):375-82. 2013.
- Yang Y, Zhang X, Zhang C. Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4. Molecular immunology. 56(1-2):64-71. 2013.
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