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GST tag antibody

Cat.#: 107309

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Product Information

  • Product Name
    GST tag antibody
  • Documents
  • Description
    GST tag Mouse Monoclonal antibody. Positive WB detected in recombinant protein. Positive IP detected in Recombinant protein protein.
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Tag
  • Alternative names
    Glutathione S-Transferase antibody; gst antibody; GST tag antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of Recombinant Protein (Accession Number: FN315687). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:5000-1:50000

    IP: 1:5000-1:50000

  • Validations

    Western blot of GST recombinant protein with anti-GST-tag (Catalog No:117321) at various dilutions.

    Western blot of GST recombinant protein with anti-GST-tag (Catalog No:117321) at various dilutions.

    IP Result of anti-GST Tag (IP:Catalog No:117321, 4ug; Detection:Catalog No:117321 1:20000) with Recombinant protein protein lysate 1000ug.

    IP Result of anti-GST Tag (IP:Catalog No:117321, 4ug; Detection:Catalog No:117321 1:20000) with Recombinant protein protein lysate 1000ug.

  • Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.
  • References
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    • Guan JJ, Zhang XD, Sun W, Qi L, Wu JC, Qin ZH. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell death & disease. 6:e1624. 2015.
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    • Liu C, Zhang D, Shen Y. DPF2 regulates OCT4 protein level and nuclear distribution. Biochimica et biophysica acta. 1853(12):3279-93. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"