-
Product Name
GSR Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to GSR
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse
-
Alternative names
GSR antibody; HEL-75 antibody; HEL-S-122m antibody; glutathione-disulfide reductase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 273-522 of human GSR (NP_000628.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - GSR Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GSR antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
Maintains high levels of reduced glutathione in the cytosol.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"