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GSPT1 antibody

Cat.#: 111175

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Product Information

  • Product Name
    GSPT1 antibody
  • Documents
  • Description
    GSPT1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in SGC-7901 cells, HeLa cells. Observed molecular weight by Western-blot: 80 kDa; 84 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    551G9.2 antibody; eRF3a antibody; ETF3A antibody; FLJ38048 antibody; FLJ39067 antibody; G1 to S phase transition 1 antibody; GSPT1 antibody; GST1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GSPT1 recombinant protein (Accession Number: NM_001130006). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:111175(GSPT1 antibody) at dilution of 1:100

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:111175(GSPT1 antibody) at dilution of 1:100

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111175(GSPT1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111175(GSPT1 antibody) at dilution of 1:100 (under 10x lens)

  • Background
    The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID:15917414,12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1.
  • References
    • Teng S, Gao L, Paajanen V, Pu J, Fan Z. Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels. Cardiovascular research. 83(3):473-80. 2009.
    • Nair S, Bora-Singhal N, Perumal D, Chellappan S. Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner. Molecular cancer. 13:173. 2014.
    • Terui Y, Sakamoto A, Yoshida T. Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA. Amino acids. 47(2):345-56. 2015.
    • Su H, Meng S, Lu Y. Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing. Molecular cell. 43(1):97-109. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"