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Product Name
GSK3B antibody
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Description
GSK3B Rabbit Polyclonal antibody. Positive WB detected in mouse ovary tissue, HEK-293 cells, HeLa cells. Positive IP detected in mouse heart tissue. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat,Pig,Zebrafish; other species not tested.
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Alternative names
GSK 3 beta antibody; GSK3 beta antibody; GSK3A B antibody; GSK3B antibody; GSK3B 216P antibody; gsk3beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSK3B recombinant protein (Accession Number: NM_002093). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:111172(GSK3B antibody) at dilution of 1:300
IP Result of anti-GSK3B (IP:Catalog No:111172, 4ug; Detection:Catalog No:111172 1:500) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111172(GSK3B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111172(GSK3B Antibody) at dilution of 1:50 (under 40x lens)
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Background
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.
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References
- Gao X, Wang JY, Gao LM, Yin XF, Liu L. Identification and analysis of glycogen synthase kinase 3 beta1 interactome. Cell biology international. 37(8):768-79. 2013.
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