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Product Name
GSK3B antibody
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Description
GSK3B Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in NIH/3T3 cells, HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 46-52kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
GSK 3 beta antibody; GSK3 beta antibody; GSK3A B antibody; GSK3B antibody; GSK-3B antibody; GSK3B 216P antibody; gsk3beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002093). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111171(GSK3B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111171(GSK3B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111171(GSK3B antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-GSK3B (IP:Catalog No:111171, 3ug; Detection:Catalog No:111171 1:500) with HeLa cells lysate 920ug.
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:111171(GSK3B Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.
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References
- Rudkouskaya A, Welch I, Dagnino L. ILK modulates epithelial polarity and matrix formation in hair follicles. Molecular biology of the cell. 25(5):620-32. 2014.
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