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Product Name
GS28 antibody
- Documents
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Description
GS28 Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Positive WB detected in human liver tissue, HepG2 cells, NIH/3T3 cells, rat liver tissue. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
28 kDa cis Golgi SNARE p28 antibody; 28 kDa Golgi SNARE protein antibody; GOLIM2 antibody; GOS 28 antibody; GOS28 antibody; GOS28/P28 antibody; GOSR1 antibody; GS28 antibody; P28 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GS28 recombinant protein (Accession Number: BC012620). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111164(GS28 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:111164(GS28 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:111164(GS28 antibody) at dilution of 1:50 (under 10x lens)
1X10^6 HeLa cells were stained with 0.2ug GS28 antibody (Catalog No:111164, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:111164(GS28 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)
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