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Product Name
GRPEL2 Polyclonal Antibody
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Description
Polyclonal antibody to GRPEL2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
GRPEL2 antibody; Mt-GrpE#2 antibody; GrpE like 2, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-225 of human GRPEL2 (NP_689620.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - GRPEL2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GRPEL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - GRPEL2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using GRPEL2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - GRPEL2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using GRPEL2 antibody at dilution of 1:100 (40x lens).
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Background
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70 (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"