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Product Name
GRPEL1 antibody
- Documents
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Description
GRPEL1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IP detected in MCF-7 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 24 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ25609 antibody; GREPEL1 antibody; GRPEL1 antibody; HMGE antibody; Mt GrpE#1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRPEL1 recombinant protein (Accession Number: NM_025196). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111224(GRPEL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111224(GRPEL1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using GRPEL1 antibody Catalog No:111224 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GRPEL1 (IP:Catalog No:111224, 4ug; Detection:Catalog No:111224 1:600) with MCF-7 cells lysate 1280ug.
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Background
GRPEL1 represents GrpE-like 1, mitochondrial (E. coli)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"