Product Name

GRP75 antibody

Documents

Description

GRP75 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue, NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, A549 cells, mouse testis tissue, rat testis tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human brain tissue. Observed molecular weight by Western-blot: 74-75 kDa

Tested applications

ELISA, WB, IHC, IF, FC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CSA antibody; GRP 75 antibody; GRP75 antibody; Heat shOCk 70 kDa protein 9 antibody; HSPA9 antibody; HSPA9B antibody; Mortalin antibody; MOT antibody; mot 2 antibody; MOT2 antibody; MTHSP75 antibody; PBP74 antibody; Peptide binding protein 74 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of GRP75 recombinant protein (Accession Number: NM_004134). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:1000-1:10000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111220(GRP75 antibody) at dilution of 1:500

A549 cells (shcontrol and shRNA of GRP75)were subjected to SDS PAGE followed by western blot with Catalog No:111220 (GRP75 antibody) at dilution of 1:500. (Data provided by Angran Biotech (www.miRNAlab.com)).

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111220(GRP75 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111220(GRP75 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:111220(GRP75 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

IP Result of anti-GRP75 (IP:Catalog No:111220, 4ug; Detection:Catalog No:111220 1:2000) with mouse brain tissue lysate 3680ug.

1X10^6 HepG2 cells were stained with 0.2ug GRP75 antibody (Catalog No:111220, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Background

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson’s disease. This antibody recognized the endogenous HSPA9 protein in NIH/3T3 cells. (21640711, 22920904)

References

Su CC, Su JH, Lin JJ. An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. Marine drugs. 9(12):2622-42. 2011.
Ho KY, Yeh TS, Huang HH. Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth. International journal of molecular sciences. 14(7):14439-59. 2013.
Richter-Dennerlein R, Korwitz A, Haag M. DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Cell metabolism. 20(1):158-71. 2014.
Korwitz A, Merkwirth C, Richter-Dennerlein R. Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria. The Journal of cell biology. 212(2):157-66. 2016.

GRP75 antibody

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