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Product Name
GRB2 antibody
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Description
GRB2 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, C2C12 cells, rat brain tissue, rat spleen tissue. Observed molecular weight by Western-blot: 25-28kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Adapter protein GRB2 antibody; ASH antibody; EGFRBP GRB2 antibody; GRB2 antibody; Grb3 3 antibody; MST084 antibody; MSTP084 antibody; Protein Ash antibody; SH2/SH3 adapter GRB2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRB2 recombinant protein (Accession Number: NM_002086). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111200(GRB2 antibody) at dilution of 1:1000
C2C12 cells (shcontrol and shRNA of GRB2) were subjected to SDS PAGE followed by western blot with Catalog No:111200(GRB2 antibody) at dilution of 1:1000. (Data provided by Angran Biotech (www.miRNAlab.com)).
Immunofluorescent analysis of HEK-293 cells using Catalog No:111200(GRB2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.
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