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Product Name
GRASP65,GORASP1 antibody
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Description
GRASP65,GORASP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 46,65 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ23443 antibody; Golgi phosphoprotein 5 antibody; GOLPH5 antibody; GORASP1 antibody; GRASP65 antibody; P65 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRASP65,GORASP1 recombinant protein (Accession Number: BC008928). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: N/AIF: 1:10-1:100
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Validations
WB result of GRASP65 anitbody (Catalog No:111196,1:1000) with HeLa cell lysate by Dr. Neeraj Tiwari, James Rothman Lab.
Immunofluorescent analysis of HeLa cells using Catalog No:111196(GRASP65,GORASP1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. (refer to Wikipedia)
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