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Product Name
GPX3 Polyclonal Antibody
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Description
Polyclonal antibody to GPX3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
GPX3 antibody; GPx-P antibody; GSHPx-3 antibody; GSHPx-P antibody; glutathione peroxidase 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human GPX3.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - GPX3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPX3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"